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The S-RAB window consists of i a Gene information view upper left ; ii an Alignment view bottom ; and iii a Short-read information view upper right. A large-scale gene expression study is a powerful tool to deepen the understanding of the gene functions of rice Hamada et al.

To illustrate the genome-wide gene expression profile derived from the next-generation sequencing technology, we incorporated 27 publicly available Illumina RNA-Seq data sets, which include seven tissues, 16 stress conditions and four normal conditions Mizuno et al.

For the stress and normal conditions, total RNAs were sampled from the roots and leaves Mizuno et al. We quantified the expression level of each transcript by calculating the number of uniquely mapped reads per kilobase per million reads RPKM and found that 33, of the 37, loci We detected expression for 2, of the 8, ab initio predicted loci without any transcriptional evidence The expression level of each gene is shown at a single nucleotide resolution in the gene map view Fig.

To provide researchers with the basic knowledge of how genes are conserved among plant species and how gene families evolve in each species, we constructed gene family data from rice and two other fully sequenced monocot species, Sorghum bicolor and Zea mays , and a dicot species, Arabidopsis thaliana.

The protein sequences of S. First, we clustered the families of homologous genes using the Markov clustering MCL program with an option of -l 1. The gene family names were determined based on their functional domains, as predicted by InterProScan e. For each gene family, we performed a BLASTP search against the UniProt and RefSeq protein databases by querying the protein sequences in the gene family to collect homologous sequences comprehensively among the plant species.

After removing the sequence redundancy, we created a multiple alignment using ClustalW Larkin et al. The trees were rooted by the mid-point rooting method using our custom-made program. A Main window of IDCGP showing the phylogenetic trees, hyperlinks to the downloadable data and buttons for adding sequences and editing multiple sequence alignments. B A window for editing multiple sequence alignments. Comparative genome analysis of a wide variety of Oryza species is expected to be more beneficial than analysis of a single species.

A previous study suggested that the genome of cultivated rice had lost a significant number of genes during domestication, whereas such lost genes were preserved in wild rice species Sakai and Itoh Its result showed that 24 primer sets successfully amplified DNA fragments; 15 of them were confirmed to be specific to O.

These O. Hence, we expect that this information will be useful for breeding in the future. The gene structures and annotations are shown on GBrowse version 2. The gene map view is based on GBrowse Fig.

The gene details view Fig. Users can query sequences by directly pasting the sequences in the text box or by uploading a file in FASTA format.

Options for filtering low-complexity sequences and avoiding lower case sequences when searching for initial exact matches and limiting the number of outputs are available as advanced options. In cases of searching DNA sequences against the genome sequence, hyperlinks to GBrowse are provided in the result page so that users can visually check where and how the query sequences match to the genome.

This function is quite useful, particularly when searching transcript sequences. The batch retrieval tool enables users to retrieve sequences or annotation data of specific IDs or chromosomal region. In addition, by specifying any two SSR markers, users can obtain the data between them, which would be helpful, for example, to search for candidate genes of the target region detected by quantitative trait locus QTL analysis Konishi et al. The S-RAB window consists of three sections: i Gene information view upper left ; ii Alignment view bottom ; and iii Short-read information view upper right.

The gene information view shows the detailed annotation information of the gene overlapping with the contig selected by the user. The information includes the functional annotation, number of mapped short reads, gene orientation and pull-down list of the SNPs. Each row in the SNP list represents the position of the SNP, nucleotide change and amino acid change, if available, from the left.

Users can view the detailed alignment of an SNP and the surrounding region in the alignment view by selecting one of the SNPs in the list. In the alignment information view, the Nipponbare reference sequence is shown on the top and is represented by four colors: red, UTRs; blue, CDSs; gray, introns; and black, intergenic regions.

The color pattern is based on the transcript selected in the gene information view and can be changed if more than one alternative variant is available. Transcripts other than the selected one are shown below the reference sequence with the same color pattern.

The SNPs are highlighted in the alignments, and amino acid changes are shown above the reference sequence, e. The alignment view does not show the reverse complement sequence, even if the selected gene resides on the reverse strand.

Therefore, in such cases, the amino acid changes are represented in reverse orientation, i. The SNPs are predicted simply based on the frequency of the variants with quality value of above the threshold. The short-read information view shows the detailed information of the read selected in the alignment view. PGFD displays lists of gene family and subfamilies.

The gene family window shows a list of the subfamilies contained in the gene family, and the subfamily window shows a list of the genes contained in the subfamily. When I type brew deps -n msodbcsql17 I see openssl 3 , where some of my teammates and I used to see openssl 1.

So now whenever I install msodbcsql17 out of the box, it overwrites openssl. We are looking into what is different with this new version of openssl. For what it's worth, and for anybody else coming here via a web search: this very same error occurs when trying to use this driver with PHP on macOS. Using the symlink change workaround mentioned by jh88 above 59 comment makes it work correctly on my system.

Followed the steps to install openssl 1. EDIT: Turns out, the issue was with the symlink. The version is actually 1. How to fix this? I am having the same issue on my MAC It did work but suddenly stopped.

I am able to connect to the server through Azure Data Studio. I have OpenSSL 1. See the temporary solution here. I don't know if three is anything else that I missed? I am not so familiar with all these Linux commands? Not sure why, but I was having the exact same problem and tried to run everything without anaconda and then the solution of 59 comment worked for me.

Working on a MBP M1 here, I tried all the workarounds listed in this issue but none of them worked for me. I successfully linked the openssl to the correct version installed by homebrew. I even uninstalled openssl 3 to make sure the version used by mssql would be the correct one. For ARM the path is slightly different.

Ah I managed to solve it thanks to you v-chojas. I only had openssl 1. Uninstall Openssl 3 and install Openssl 1. Read, enjoy, and share! No fee or registration! Everything from Project Gutenberg is gratis, libre, and completely without cost to readers. If you find Project Gutenberg useful, please consider a small donation to help Project Gutenberg digitize more books, maintain its online presence, and improve Project Gutenberg programs and offerings.

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Alex Mamo Alex Mamo k 14 14 gold badges silver badges bronze badges. Using this code, the application no longer crashes. When you press login, the page closes but the app does not move forward, it goes back to the starting page. In which place of your activity are you sing thise code? Can you share it? Is onDataChange even called?

I see now but you aren't using my entire answer. Your DatabaseReference is wrong. Does it work now? Show 11 more comments. Ron Daulagupu Ron Daulagupu 4 4 silver badges 16 16 bronze badges.